PXD031227 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Reconstitution of 3’-processing of mammalian pre-mRNA reveals a central role of RBBP6 |
Description | The 3’ ends of almost all eukaryotic mRNAs are generated in an essential two-step processing reaction, endonucleolytic cleavage of an extended precursor followed by the addition of a poly(A) tail. By reconstituting the reaction from overproduced and purified proteins, we provide a minimal list of fourteen polypeptides essential and two stimulatory for RNA processing. In a reaction depending on the polyadenylation signal AAUAAA, the reconstituted system cleaves pre-mRNA at a single preferred site corresponding to the one used in vivo. Among the proteins, Cleavage Factor I stimulates cleavage but is not essential, consistent with its prominent role in alternative polyadenylation. RBBP6 is required, structural data showing it to contact and presumably activate the endonuclease CPSF73 through its DWNN domain. The C-terminal domain of RNA polymerase II is dispensable. ATP, but not its hydrolysis, supports RNA cleavage by binding to the hClp1 subunit of cleavage factor II with submicromolar affinity. |
HostingRepository | PRIDE |
AnnounceDate | 2022-03-04 |
AnnouncementXML | Submission_2022-03-04_04:52:22.746.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Florian Kluge |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-25 00:54:58 | ID requested | |
⏵ 1 | 2022-03-04 04:52:23 | announced | |
Publication List
Schmidt M, Kluge F, Sandmeir F, K, ü, hn U, Sch, ä, fer P, T, ü, ting C, Ihling C, Conti E, Wahle E, Reconstitution of 3' end processing of mammalian pre-mRNA reveals a central role of RBBP6. Genes Dev, 36(3-4):195-209(2022) [pubmed] |
Keyword List
submitter keyword: XL-MS , 3’-processing , RBBP6 , CPSF |
Contact List
Elmar Wahle |
contact affiliation | University of Halle Department of Biochemistry Germany |
contact email | ewahle@biochemtech.uni-halle.de |
lab head | |
Florian Kluge |
contact affiliation | Institute for Biochemistry and Biotechnology; AG Wahle |
contact email | florian.kluge@bct.uni-halle.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031227
- Label: PRIDE project
- Name: Reconstitution of 3’-processing of mammalian pre-mRNA reveals a central role of RBBP6