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PXD031222-1

PXD031222 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAugmented enterocyte damage during Candida albicans and Proteus mirabilis coinfection
DescriptionThe human gut acts as the main reservoir of microbes and a relevant source of life-threatening infections, especially in immunocompromised patients. There, the opportunistic fungal pathogen Candida albicans adapts to the host environment and additionally interacts with residing bacteria. We investigated fungal-bacterial interactions by coinfecting enterocytes with the yeast Candida albicans and the Gram-negative bacterium Proteus mirabilis resulting in enhanced host cell damage. This synergistic effect was conserved across different P. mirabilis isolates and occurred also with non-albicans Candida species and C. albicans mutants defective in filamentation or candidalysin production. Using bacterial deletion mutants, we identified the P. mirabilis hemolysin HpmA to be the key effector for host cell destruction. Spatially separated coinfections demonstrated that synergism between Candida and Proteus is induced by contact, but also by soluble factors. Specifically, we identified Candida-mediated glucose consumption and farnesol production as potential triggers for Proteus virulence. In summary, our study demonstrates that coinfection of enterocytes with C. albicans and P. mirabilis can result in increased host cell damage which is mediated by bacterial virulence factors as a result of fungal niche modification via nutrient consumption and production of soluble factors. This supports the notion that certain fungal-bacterial combinations have the potential to result in enhanced virulence in niches such as the gut and might therefore promote translocation and dissemination.
HostingRepositoryPRIDE
AnnounceDate2022-05-09
AnnouncementXMLSubmission_2022-05-09_08:01:19.810.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThomas Krüger
SpeciesList scientific name: Candida albicans (Yeast); NCBI TaxID: 5476; scientific name: Proteus mirabilis HI4320; NCBI TaxID: 529507;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-25 00:46:55ID requested
12022-05-09 08:01:20announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Candida albicans, Proteus mirabilis, coinfection, synergism, cross-kingdom interaction, enterocytes
Contact List
Axel A. Brakhage
contact affiliationDepartment of Molecular and Applied Microbiology Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute Jena, Germany
contact emailaxel.brakhage@leibniz-hki.de
lab head
Thomas Krüger
contact affiliationLeibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact emailthomas.krueger@leibniz-hki.de
dataset submitter
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Dataset FTP location
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