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PXD031136-2

PXD031136 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDecoupling astrocytes in adult mice impairs synaptic plasticity and spatial learning
DescriptionTo study the role of the gap junction coupled astrocytic network, we generated inducible double knockouts to selectively delete Cx30 and Cx43 from astrocytes in adult mice. Mice carrying loxP-flanked Gjb6 (Cx30fl/fl mice) (Boulay et al., 2013) and Gja1 (Cx43fl/fl mice) (Theis et al., 2003) alleles were crossbred with mice expressing the tamoxifen-sensitive Cre-recombinase CreERT2 under the endogenous GLAST(Slc1a3)-promoter (Mori et al., 2006). Adult, 8-10 week old mice (Cx30fl/fl:Cx43fl/fl:GLASTCreERT2/+, termed cKO) and littermate control mice (Cx30fl/fl:Cx43fl/fl:GLAST+/+) were injected with tamoxifen for 5 consecutive days and hippocampi were isolated for subsequent TMT-based proteomics analysis 90 days after tamoxifen treatment, to study the consequences of astrocyte decoupling and connexin deletion.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:55:20.599.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAiman Saab
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-21 07:19:29ID requested
12022-03-14 04:13:03announced
22023-11-14 08:55:21announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: LC-MSMS, connexin 43, astrocytes, connexin 30, hippocampus,Mouse, TMT proteomics
Contact List
Aiman Saab
contact affiliationUniversity of Zurich, Institute of Pharmacology and Toxicology, Zurich, Switzerland
contact emailasaab@pharma.uzh.ch
lab head
Aiman Saab
contact affiliationUniversity of Zurich, Institute of Pharmacology & Toxicology
contact emailasaab@pharma.uzh.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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