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PXD031123-2

PXD031123 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA seed-like proteome in oil-rich tubers
DescriptionThere are numerous examples in plants, where certain organs or developmental stages are desiccation tolerant and can withstand extended periods of severe water loss. One prime example are seeds and pollen of many spermatophytes. However, in some plants, also vegetative organs can be desiccation tolerant as for example the tubers of yellow nutsedge (Cyperus esculentus) that also store larger amounts of lipids similar to seeds. Interestingly, the closest relative purple nutsedge (Cyperus rotundus) generates tubers that do not accumulate oil and are not desiccation tolerant. We generated nanoLC-MS/MS-based proteomes of yellow nutsedge in five replicates of four stages of tuber development and compared them to the proteomes of roots and leaves, yielding 2257 distinct protein groups. Our data reveal a striking upregulation of hallmark proteins of seeds in the tubers. A deeper comparison to the tuber proteome of the closest relative purple nutsedge (Cyperus rotundus) and a previously published proteome of Arabidopsis seeds and seedlings indicates that indeed a seed-like proteome was found in yellow but not purple nutsedge. This was further supported by an analysis of the proteome of a lipid-droplet enriched fraction of yellow nutsedge, which also displayed seed-like characteristics. One reason for the differences between the two nutsedge species might be the expression of certain transcription factors homolog to ABSCISIC ACID INSENSITIVE3, WRINKLED1 and LEAFY COTYLEDON1 that drive gene expression in Arabidopsis seed embryos.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:54:42.785.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTill Ischebeck
SpeciesList scientific name: Cyperus rotundus; NCBI TaxID: 512623;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-20 09:27:19ID requested
12022-10-31 09:17:55announced
22023-11-14 08:54:43announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Yellow nutsedge, Cyperus esculentus, tubers
Contact List
Till Ischebeck
contact affiliationWestfälische Wilhelms-Universität Münster Institut für Biologie und Biotechnologie der Pflanzen Arbeitsgruppe Grüne Biotechnologie Schlossplatz 8 48143 Münster
contact emailtill.ischebeck@uni-muenster.de
lab head
Till Ischebeck
contact affiliationUniversity of Münster
contact emailtischeb@gwdg.de
dataset submitter
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Dataset FTP location
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