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PXD031072-1

PXD031072 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA plant tethering system for the functional study of protein-RNA interactions in vivo
DescriptionThe sorting of RNA transcripts dictates their ultimate post-transcriptional fates, such as translation, decay or degradation by RNA interference (RNAi). This sorting of RNAs into distinct fates is mediated by their interaction with RNA-binding proteins. While hundreds of RNA binding proteins have been identified, which act to sort RNAs into different pathways is largely unknown. Particularly in plants, this is due to the lack of reliable protein-RNA artificial tethering tools necessary to determine the mechanism of protein action on an RNA in vivo. Here we used minimal Arabidopsis BRN1 RNA binding domains to generate a protein-RNA tethering system which functions on an endogenous Arabidopsis RNA called SOC1 that is tracked by the quantitative flowering time phenotype. Unlike other protein-RNA tethering systems that have been attempted in plants, our system circumvents the inadvertent triggering of RNAi. We successfully in vivo tethered a protein epitope to the RNA and identified new proteins that were previously unknown to interact during BRN1-RNA binding. This protein-RNA tethering system enables the future determination of any protein’s function upon recruitment to an RNA and can be used to discover new interactions with RNA-binding proteins.
HostingRepositoryPRIDE
AnnounceDate2022-05-10
AnnouncementXMLSubmission_2022-05-09_16:23:30.755.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD031072
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterYu-Hung Hung
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-19 03:01:02ID requested
12022-05-09 16:23:31announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Arabidopsis, RNA binding, IP-MS, Bruno1
Contact List
R. Keith Slotkin
contact affiliationDonald Danforth Plant Science Center, St. Louis, MO, USA Division of Biological Sciences, University of Missouri, Columbia, MO, USA
contact emailKSlotkin@danforthcenter.org
lab head
Yu-Hung Hung
contact affiliationDonald Danforth Plant Science Center
contact emailyhung@danforthcenter.org
dataset submitter
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Dataset FTP location
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