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PXD031063-1

PXD031063 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleABP1-TMK auxin perception mediates rapid phosphorylation for regeneration and auxin canalization
DescriptionThe phytohormone auxin triggers transcriptional reprograming utilizing a well-characterized nuclear perception machinery. In contrast, mechanisms underlying other auxin effects, such as auxin feed-back on its transport, rapid regulation of ion fluxes or ultrafast global phospho-response, remain enigmatic. Auxin Binding Protein 1 (ABP1) has been contested as an auxin receptor candidate since decades. Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds auxin specifically at the acidic pH typical for the apoplast. ABP1 and its plasma membrane-localized partner, Transmembrane Kinase 1 (TMK1) are required for auxin-induced phosphorylation of about thousand proteins. Loss-of-function abp1 alleles but not complemented lines show defects in in vitro shoot regeneration and formation of auxin-transporting channels for vasculature formation and regeneration. These results support a role of ABP1-TMK1 in cell surface auxin perception mediating global auxin phospho-response and regenerative development.
HostingRepositoryPRIDE
AnnounceDate2022-08-25
AnnouncementXMLSubmission_2022-08-25_03:50:37.739.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMark Roosjen
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListphosphorylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-19 02:36:06ID requested
12022-08-25 03:50:38announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Auxin ABP1 TMK1 phosphoproteomics
Contact List
Prof.Dr. Dolf Weijers
contact affiliationDepartment head
contact emaildolf.weijers@wur.nl
lab head
Mark Roosjen
contact affiliationWageningen University and Research
contact emailmark.roosjen@wur.nl
dataset submitter
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Dataset FTP location
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