PXD031063

PXD031063 is an original dataset announced via ProteomeXchange.

Title	ABP1-TMK auxin perception mediates rapid phosphorylation for regeneration and auxin canalization
Description	The phytohormone auxin triggers transcriptional reprograming utilizing a well-characterized nuclear perception machinery. In contrast, mechanisms underlying other auxin effects, such as auxin feed-back on its transport, rapid regulation of ion fluxes or ultrafast global phospho-response, remain enigmatic. Auxin Binding Protein 1 (ABP1) has been contested as an auxin receptor candidate since decades. Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds auxin specifically at the acidic pH typical for the apoplast. ABP1 and its plasma membrane-localized partner, Transmembrane Kinase 1 (TMK1) are required for auxin-induced phosphorylation of about thousand proteins. Loss-of-function abp1 alleles but not complemented lines show defects in in vitro shoot regeneration and formation of auxin-transporting channels for vasculature formation and regeneration. These results support a role of ABP1-TMK1 in cell surface auxin perception mediating global auxin phospho-response and regenerative development.
HostingRepository	PRIDE
AnnounceDate	2022-08-25
AnnouncementXML	Submission_2022-08-25_03:50:37.739.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mark Roosjen
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-19 02:36:06	ID requested
⏵ 1	2022-08-25 03:50:38	announced

Dataset with its publication pending

submitter keyword: Auxin ABP1 TMK1 phosphoproteomics

Prof.Dr. Dolf Weijers
contact affiliation	Department head
contact email	dolf.weijers@wur.nl
lab head
Mark Roosjen
contact affiliation	Wageningen University and Research
contact email	mark.roosjen@wur.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031063
     1. Label: PRIDE project
     2. Name: ABP1-TMK auxin perception mediates rapid phosphorylation for regeneration and auxin canalization