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PXD031054-2

PXD031054 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomics and in-cell cross-linking reveal cellular reorganisation during early neuronal differentiation of SH-SY5Y cells
DescriptionThe proteomes of undifferentiated and differentiated SH-SY5Y cells are characterised and compared. For this, neuronal differentiation using retinoic acid (RA) or a combination of RA and phorbol-12-myristat-13-acetate (RA/PMA) was explored. An MS-based label-free quantification approach is applied to identify changes in the protein expression, the as proteins’ subcellular localisation abundance as well as their association with enriched KEGG pathways. By employing formaldehyde cross-linking insights into protein interaction networks of undifferentiated as well as RA- and RA/PMA-differentiated neurons are obtained. The analyses provided insights into the proteomes of undifferentiated and differentiated SH-SY5Y cells and suggest structural rearrangements, for instance, of the actin network during neuronal differentiation.
HostingRepositoryPRIDE
AnnounceDate2022-04-08
AnnouncementXMLSubmission_2022-04-08_08:41:09.594.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarie Barth
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-18 02:32:12ID requested
12022-04-08 03:12:33announced
22022-04-08 08:41:09announced2022-04-08: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proteomics, chemical cross-linking, mass spectrometry, protein networks, actin, SH-SY5Y
Contact List
Carla Schmidt
contact affiliationInterdisciplinary research center HALOmem, Institute of Biochemistry and Biotechnology, Charles Tanford Protein Center, Martin Luther University Halle-Wittenberg, Halle, Germany
contact emailcarla.schmidt@biochemtech.uni-halle.de
lab head
Marie Barth
contact affiliationHALOmem, Martin Luther University Halle-Wittenberg
contact emailmarie.barth@bct.uni-halle.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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