PXD030933 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Hypoxia-induced subnuclear re-distribution of SAFB1/2 alters their respective interactome |
Description | Oxygen deprivation (hypoxia) is encountered in physiological conditions but also characterizes many pathological conditions including ischemia and cancer. In order to survive, the cells usually rely upon the activation of the Hypoxia Inducible Factors (HIF), a small family of heterodimeric transcriptional activators. However, adaption to hypoxia also involves, lesser-known, HIF-independent processes that affect chromatin remodelling and nuclear architecture. SAFB1/2 (Scaffold Attachment Factors B1 and 2) are integral components of the nuclear matrix of vertebrate cells and known to affect functions that include transcriptional regulation, RNA processing and response to DNA damage. Our work indicates that SAFB1/2 swiftly change their localization from the insoluble matrix fraction to more soluble nuclear fractions in a HIF-independent manner. To extend our study, we performed immunoprecipitation experiments with SAFB1 or SAFB2 from cells exposed to both normoxia and short-term hypoxia (2 hours). Mass spectrometry analysis of the immunoprecipitates revealed that short-term exposure of cells to low oxygen levels alters the protein-protein interactions of SAFB1/2 mainly with proteins implicated in mRNA maturation and transcriptional control. Our data suggest that SAFB1/2 proteins participate in molecular mechanisms immediately responsive to hypoxia that entail their rapid mobilization from the nuclear matrix and their altered association with constituents of splicing and transcriptional machinery. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:17:22.898.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Martina Samiotaki |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap XL |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-13 02:52:26 | ID requested | |
1 | 2023-03-10 13:24:44 | announced | |
⏵ 2 | 2023-11-14 08:17:26 | announced | 2023-11-14: Updated project metadata. |
Publication List
Taze C, Drakouli S, Samiotaki M, Panayotou G, Simos G, Georgatsou E, Mylonis I, Short-term hypoxia triggers ROS and SAFB mediated nuclear matrix and mRNA splicing remodeling. Redox Biol, 58():102545(2022) [pubmed] |
Keyword List
submitter keyword: SAFB2, Nuclear Matrix, Splicing,Hypoxia, Scaffold Attachment Factor, SAFB1 |
Contact List
Ilias Mylonis |
contact affiliation | Laboratory of Biochemistry, Faculty of Medicine, University of Thessaly, 41500 Biopolis, Larissa, Greece |
contact email | mylonis@med.uth.gr |
lab head | |
Martina Samiotaki |
contact affiliation | Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece |
contact email | samiotaki@fleming.gr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD030933 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030933
- Label: PRIDE project
- Name: Hypoxia-induced subnuclear re-distribution of SAFB1/2 alters their respective interactome