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PXD030915-1

PXD030915 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSpatiotemporal profiling of the bovine oviduct fluid proteome around the time of ovulation
DescriptionUnraveling the composition and regulation of the oviduct fluid (OF) is crucial to understand the microenvironment in which sperm capacitation, fertilization and early embryo development take place. Therefore, our aim was to determine the spatiotemporal changes in the OF proteome according to the anatomical region of the oviduct (ampulla vs. isthmus), the proximity of the ovulating ovary (ipsilateral vs. contralateral side) and the peri-ovulatory stage (pre-ovulatory or Pre-ov vs. post-ovulatory or Post-ov). Oviducts from adult cyclic cows were collected at a local slaughterhouse and pools of OF were analyzed by nanoLC-MS/MS and label-free protein quantification (n=32 OF pools for all region  stage  side conditions). A total of 3,760 proteins were identified in the OF, of which 37% were predicted to be secreted. The oviduct region was the major source of variation in protein abundance, followed by the proximity of the ovulating ovary and finally the peri-ovulatory stage. Differentially abundant proteins between regions, stages and sides were involved in a broad variety of biological functions, including protein binding, response to stress, cell-to-cell adhesion, calcium homeostasis and the immune system. This work highlights the dynamic regulation of oviduct secretions and provides new protein candidates for interactions between the maternal environment, the gametes and the early embryo.
HostingRepositoryPRIDE
AnnounceDate2022-03-03
AnnouncementXMLSubmission_2022-03-03_03:38:12.904.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD030915
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterE Com
SpeciesList scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-12 08:59:19ID requested
12022-03-03 03:38:13announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: oviduct fluid, proteomics, mass spectrometry, ampulla, isthmus, pre-ovulatory, post-ovulatory, ipsilateral, contralateral, cattle
Contact List
Charles Pineau
contact affiliationUMS Biosit - CNRS 3480 - Inserm 018 Campus de Beaulieu - Bâtiment. 24 - CS2407 263 Avenue du Général Leclerc 35042 RENNES CEDEX FRANCE
contact emailproteome@univ-rennes1.fr
lab head
E Com
contact affiliationProtim, Irset, Inserm U1085
contact emailemmanuelle.com@univ-rennes1.fr
dataset submitter
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Dataset FTP location
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