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PXD030886-1

PXD030886 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic and functional analyses of the periodic membrane skeleton in neurons
DescriptionActin, spectrin, and associated proteins form a membrane-associated periodic skeleton (MPS) in neurons. The molecular composition and functions of the MPS remain incompletely understood. Here, we combined co-immunoprecipitation and mass spectrometry analyses to identify candidate MPS-interacting proteins from cultured hippocampal neurons and adult mouse brains. In addition, we also used quantitative mass spectrometry analysis based on Tandem Mass Tag (TMT) isobaric labeling to determine how the expression levels of proteins are differentially regulated at the proteomic scale upon depletion of βII-spectrin, an important molecular component of the MPS. These results provide a systematic picture of the interactome of the MPS, and new insights into new functions of the MPS in neurons.
HostingRepositoryPRIDE
AnnounceDate2022-05-04
AnnouncementXMLSubmission_2022-05-04_04:41:45.297.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRuobo Zhou
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-11 01:26:24ID requested
12022-05-04 04:41:45announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Actin, spectrin, membrane skeleton, neuron, proteomics
Contact List
Xiaowei Zhuang
contact affiliationHoward Hughes Medical Institute, Department of Chemistry and Chemical Biology, Department of Physics, Harvard University, Cambridge, MA 02138, USA
contact emailzhuang@chemistry.harvard.edu
lab head
Ruobo Zhou
contact affiliationThe Pennsylvania State University
contact emailruobo.zhou@psu.edu
dataset submitter
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Dataset FTP location
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