PXD030882

PXD030882 is an original dataset announced via ProteomeXchange.

Title	EVH1 domain interactors of VASP in mature and immature dendritic cells
Description	Mass spectrometry study of interactor binding to the EVH1 domain of VASP in mature and immature dendritic cells. We performed mass spectrometry study using a specially designed inhibitor that recognizes this characterized motif with high affinity and analyzed the digested peptides by LC-MS. For both, iDCs and mDCs, two independent experiments were carried out (N=2). As control for the specificity of the identified interactions an additional two independent experiments (N=2) were carried out for both, iDCs and mDCs, where a specific inhibitor of the EVH1 domain was added to outcompete bona fide EVH1 domain interactions.
HostingRepository	PRIDE
AnnounceDate	2022-10-20
AnnouncementXML	Submission_2022-10-20_03:31:22.468.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HeikeStephanowitz
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-11 01:18:06	ID requested
⏵ 1	2022-10-20 03:31:22	announced

Dataset with its publication pending

submitter keyword: dendritic cells, cell migration, actin nucleators, actin dynamics

TanjaMaritzen
contact affiliation	Department of Nanophysiology Technische Universität Kaiserslautern Paul-Ehrlich-Strasse 23 (Room 151) 67663 Kaiserslautern, Germany
contact email	maritzen@bio.uni-kl.de
lab head
HeikeStephanowitz
contact affiliation	Leibniz-Forschungsinstitut für Molekulare Pharmakologie
contact email	stephanowitz@fmp-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD030882

PRIDE project URI

• PRIDE
  1. PXD030882
     1. Label: PRIDE project
     2. Name: EVH1 domain interactors of VASP in mature and immature dendritic cells