Mass spectrometry study of interactor binding to the EVH1 domain of VASP in mature and immature dendritic cells. We performed mass spectrometry study using a specially designed inhibitor that recognizes this characterized motif with high affinity and analyzed the digested peptides by LC-MS. For both, iDCs and mDCs, two independent experiments were carried out (N=2). As control for the specificity of the identified interactions an additional two independent experiments (N=2) were carried out for both, iDCs and mDCs, where a specific inhibitor of the EVH1 domain was added to outcompete bona fide EVH1 domain interactions.