PXD030778-1

PXD030778 is an original dataset announced via ProteomeXchange.

Title	∆glnA Enterococcus faecalis V583 - data analysis
Description	A group of gram positive bacteria that share the characteristic of fermenting hexose sugars to lactic acid are generally referred to as lactic acid bacteria (LAB). Enterococcus faecalis is one of the widely studied LABs due to a multiutude of reasons. On the one hand, it plays an important role in dairy industry, being for example a starter in cheese cultures. On the other hand, it accounts for a large part of the infections caused by the LABs in hospital environments. During the past few years, it developed resistance against most of the major antibiotics. Here, in an attempt to study its adaptive metabolism, a glutamine synthetase mutant (∆glnA) of E. faecalis was subjected to pH shift and the results from the integrative analysis of its metabolic network were compared to those of the wild type. The proteome data generated in this study were used to constrain the genome-scale metabolic network at two pH level, aiming to reduce the solution space and improve the accuracy of model simulation. This data particularly helped to come up with a new design for the amino acid transport system in the genome-scale model, resulting in an accurate reproduction of the metabolic behaviour of E. faecalis.
HostingRepository	PRIDE
AnnounceDate	2022-01-14
AnnouncementXML	Submission_2022-01-14_03:07:28.022.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ludovic Gillet
SpeciesList	scientific name: Enterococcus faecalis (Streptococcus faecalis); NCBI TaxID: 1351;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-06 01:44:39	ID requested
⏵ 1	2022-01-14 03:07:28	announced

Dataset with its publication pending

submitter keyword: ∆glnA, Enterococcus faecalis, proteomics, SWATH

Ursula Kummer
contact affiliation	Department of Modeling of Biological Processes COS, Heidelberg University, Germany
contact email	ursula.kummer@bioquant.uni-heidelberg.de
lab head
Ludovic Gillet
contact affiliation	ETH Zurich
contact email	gillet@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD030778

PRIDE project URI

• PRIDE
  1. PXD030778
     1. Label: PRIDE project
     2. Name: ∆glnA Enterococcus faecalis V583 - data analysis