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PXD030778

PXD030778 is an original dataset announced via ProteomeXchange.

Dataset Summary
Title∆glnA Enterococcus faecalis V583 - data analysis
DescriptionA group of gram positive bacteria that share the characteristic of fermenting hexose sugars to lactic acid are generally referred to as lactic acid bacteria (LAB). Enterococcus faecalis is one of the widely studied LABs due to a multiutude of reasons. On the one hand, it plays an important role in dairy industry, being for example a starter in cheese cultures. On the other hand, it accounts for a large part of the infections caused by the LABs in hospital environments. During the past few years, it developed resistance against most of the major antibiotics. Here, in an attempt to study its adaptive metabolism, a glutamine synthetase mutant (∆glnA) of E. faecalis was subjected to pH shift and the results from the integrative analysis of its metabolic network were compared to those of the wild type. The proteome data generated in this study were used to constrain the genome-scale metabolic network at two pH level, aiming to reduce the solution space and improve the accuracy of model simulation. This data particularly helped to come up with a new design for the amino acid transport system in the genome-scale model, resulting in an accurate reproduction of the metabolic behaviour of E. faecalis.
HostingRepositoryPRIDE
AnnounceDate2022-01-14
AnnouncementXMLSubmission_2022-01-14_03:07:28.022.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLudovic Gillet
SpeciesList scientific name: Enterococcus faecalis (Streptococcus faecalis); NCBI TaxID: 1351;
ModificationListiodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-06 01:44:39ID requested
12022-01-14 03:07:28announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: ∆glnA, Enterococcus faecalis, proteomics, SWATH
Contact List
Ursula Kummer
contact affiliationDepartment of Modeling of Biological Processes COS, Heidelberg University, Germany
contact emailursula.kummer@bioquant.uni-heidelberg.de
lab head
Ludovic Gillet
contact affiliationETH Zurich
contact emailgillet@imsb.biol.ethz.ch
dataset submitter
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Dataset FTP location
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