PXD030726 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Arsenic toxicity is regulated by queuine availability and oxidation-induced reprogramming of the human tRNA epitranscriptome |
Description | Cells respond to environmental stress by regulating gene expression at the level of both transcription and translation. The ~50 modified ribonucleotides of the human epitranscriptome contribute to the latter, with mounting evidence that dynamic regulation of tRNA wobble modifications leads to selective translation of stress response proteins from codon-biased genes. Here we show that the response of human HepG2 cells to arsenite exposure is regulated by the availability of queuine, a micronutrient and essential precursor to the wobble modification queuosine (Q) on tRNAs reading GUN codons. Among oxidizing and alkylating agents at equitoxic concentrations, arsenite exposure caused an oxidant-specific increase in Q that correlated with up-regulation of proteins from codon-biased genes involved in energy metabolism. Limiting queuine increased arsenite-induced cell death, altered translation, increased reactive oxygen species levels, and caused mitochondrial dysfunction. In addition to revealing a new epitranscriptomic facet of arsenite toxicity and response, our results highlight the mechanistic links between environmental exposures, stress tolerance, and micronutrients. |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-27 |
AnnouncementXML | Submission_2022-08-27_03:38:12.411.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Peter Dedon |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-05 01:29:49 | ID requested | |
⏵ 1 | 2022-08-27 03:38:12 | announced | |
2 | 2023-11-14 08:54:12 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Human HepG2 arsenic epitranascriptome proteome |
Contact List
Peter Dedon |
contact affiliation | Massachusetts Institute of Technology |
contact email | pcdedon@mit.edu |
lab head | |
Peter Dedon |
contact affiliation | Massachusetts Institute of Technology |
contact email | pcdedon@mit.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD030726 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030726
- Label: PRIDE project
- Name: Arsenic toxicity is regulated by queuine availability and oxidation-induced reprogramming of the human tRNA epitranscriptome