PXD030723 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic profiling of idiopathic Parkinson’s disease primary patient cells by SWATH-MS |
Description | We performed Sequential Window Acquisition of all THeoretical Mass Spectra (SWATH-MS) on primary patient olfactory neuroepithelial derived cells (ONS) from both idiopathic PD (iPD) and healthy controls and identified 228 differentially quantified proteins. Reactome pathway analysis revealed that the “Neutrophil degranulation and XBP1(S) activates chaperone genes pathways” were the most affected, indicating disruption of the secretory pathway. Upon closer inspection we identified that proteins associated with the endoplasmic reticulum and the secretory pathways were the most abundantly changed, in particular proteins associated with the unfolded protein response (UPR). In order to validate this finding, we performed qRT-PCR analysis on patient ONS subjected to ER stressors and confirmed that the iPD patient cells had elevated UPR responses, in particular to tunicamycin mediated ER stress. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-20 |
AnnouncementXML | Submission_2022-05-20_05:10:24.294.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Peter Hains |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-05 01:17:09 | ID requested | |
⏵ 1 | 2022-05-20 05:10:25 | announced | |
Publication List
Wood SA, Hains PG, Muller A, Hill M, Premarathne S, Murtaza M, Robinson PJ, Mellick GD, Sykes AM, Proteomic profiling of idiopathic Parkinson's disease primary patient cells by SWATH-MS. Proteomics Clin Appl, 16(5):e2200015(2022) [pubmed] |
Keyword List
submitter keyword: LC-MS/MS, SWATH, Parkinson's |
Contact List
Peter Hains |
contact affiliation | Children's Medical Research Institute |
contact email | phains@cmri.org.au |
lab head | |
Peter Hains |
contact affiliation | Children's Medical Research Institute |
contact email | phains@cmri.org.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD030723 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030723
- Label: PRIDE project
- Name: Proteomic profiling of idiopathic Parkinson’s disease primary patient cells by SWATH-MS