PXD030723

PXD030723 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of idiopathic Parkinson’s disease primary patient cells by SWATH-MS
Description	We performed Sequential Window Acquisition of all THeoretical Mass Spectra (SWATH-MS) on primary patient olfactory neuroepithelial derived cells (ONS) from both idiopathic PD (iPD) and healthy controls and identified 228 differentially quantified proteins. Reactome pathway analysis revealed that the “Neutrophil degranulation and XBP1(S) activates chaperone genes pathways” were the most affected, indicating disruption of the secretory pathway. Upon closer inspection we identified that proteins associated with the endoplasmic reticulum and the secretory pathways were the most abundantly changed, in particular proteins associated with the unfolded protein response (UPR). In order to validate this finding, we performed qRT-PCR analysis on patient ONS subjected to ER stressors and confirmed that the iPD patient cells had elevated UPR responses, in particular to tunicamycin mediated ER stress.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_05:10:24.294.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Hains
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-05 01:17:09	ID requested
⏵ 1	2022-05-20 05:10:25	announced

Wood SA, Hains PG, Muller A, Hill M, Premarathne S, Murtaza M, Robinson PJ, Mellick GD, Sykes AM, Proteomic profiling of idiopathic Parkinson's disease primary patient cells by SWATH-MS. Proteomics Clin Appl, 16(5):e2200015(2022) [pubmed]

submitter keyword: LC-MS/MS, SWATH, Parkinson's

Peter Hains
contact affiliation	Children's Medical Research Institute
contact email	phains@cmri.org.au
lab head
Peter Hains
contact affiliation	Children's Medical Research Institute
contact email	phains@cmri.org.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD030723

PRIDE project URI

• PRIDE
  1. PXD030723
     1. Label: PRIDE project
     2. Name: Proteomic profiling of idiopathic Parkinson’s disease primary patient cells by SWATH-MS