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PXD030532-2

PXD030532 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCross-linking of the Endolysosomal System Reveals Potential Flotillin Structures and Cargo
DescriptionLysosomes are well-established as the main cellular organelles for the degradation of macromolecules and emerging as regulatory centers of metabolism. They are of crucial importance for cellular homeostasis, which is exemplified by a plethora of disorders related to alterations in lysosomal function. In this context, protein complexes play a decisive role, regulating not only metabolic lysosomal processes but also lysosome biogenesis, transport, and interaction with other organelles. Using cross-linking mass spectrometry, we analyzed lysosomes and early endosomes. Based on the identification of 5,376 cross-links, we investigated protein-protein interactions and structures of lysosome- and endosome-related proteins. In particular, we present evidence for a tetrameric assembly of the lysosomal hydrolase PPT1 and a heterodimeric structure of FLOT1/FLOT2 at lysosomes and early endosomes. For FLOT1-/FLOT2-positive early endosomes, we identified >300 proteins presenting putative cargo, and confirmed several substrates for flotillin-dependent endocytosis, including the latrophilin family of adhesion G protein-coupled receptors.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:54:29.467.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJasjot Singh
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-20 01:48:22ID requested
12022-11-29 02:47:46announced
22023-11-14 08:54:29announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Lysosomal interactome, cross-linking MS
Contact List
Dominic Winter
contact affiliationWinter Lab Institute for Biochemistry and Molecular Biology (IBMB) Nussallee 11 53115 Bonn, Germanz
contact emaildwin@uni-bonn.de
lab head
Jasjot Singh
contact affiliationUniversity of Bonn
contact emailjasjotsingh@web.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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