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PXD030467-1

PXD030467 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFunctional Requirements for a Samd14-Capping Protein Complex in Stress Erythropoiesis
DescriptionAcute anemia induces rapid expansion of erythroid precursors and accelerated differentiation to replenish erythrocytes. Paracrine signals – involving cooperation between SCF/c-Kit signaling and other signaling inputs – are required for the activation and function of erythroid precursors in anemia. Our prior work revealed that the Sterile Alpha Motif (SAM) Domain 14 (Samd14) gene is transcriptionally upregulated in a model of acute anemia. Samd14 expression increases the regenerative capacity of the erythroid system and promotes stress-dependent c-Kit signaling. However, the mechanism underlying Samd14’s role in stress erythropoiesis is unknown. We identified a protein-protein interaction between Samd14 and the α- and β heterodimers of the F-actin capping protein (CP) complex. Knockdown of the CP β subunit increased erythroid maturation in ex vivo cultures and decreased colony forming potential of stress erythroid precursors. In a genetic complementation assay for Samd14 activity, our results revealed that the Samd14-CP interaction is a determinant of erythroid precursor cell levels and function. Samd14-CP promotes SCF/c-kit signaling in CD71med spleen erythroid precursors.
HostingRepositoryPRIDE
AnnounceDate2022-08-09
AnnouncementXMLSubmission_2022-08-09_07:51:03.167.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD030467
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSophe Alvarez
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue; monohydroxylated residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-16 12:33:55ID requested
12022-08-09 07:51:03announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Samd14
capping protein
c-Kit
erythropoietin
stress erythropoiesis
Contact List
Sophie Alvarez
contact affiliationProteomics and Metabolomics Facility, University of Nebraska- Lincoln
contact emailsalvarez@unl.edu
lab head
Sophe Alvarez
contact affiliationUniversity of Nebraska - Lincoln
contact emailsalvarez@unl.edu
dataset submitter
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Dataset FTP location
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