PXD030467

PXD030467 is an original dataset announced via ProteomeXchange.

Title	Functional Requirements for a Samd14-Capping Protein Complex in Stress Erythropoiesis
Description	Acute anemia induces rapid expansion of erythroid precursors and accelerated differentiation to replenish erythrocytes. Paracrine signals – involving cooperation between SCF/c-Kit signaling and other signaling inputs – are required for the activation and function of erythroid precursors in anemia. Our prior work revealed that the Sterile Alpha Motif (SAM) Domain 14 (Samd14) gene is transcriptionally upregulated in a model of acute anemia. Samd14 expression increases the regenerative capacity of the erythroid system and promotes stress-dependent c-Kit signaling. However, the mechanism underlying Samd14’s role in stress erythropoiesis is unknown. We identified a protein-protein interaction between Samd14 and the α- and β heterodimers of the F-actin capping protein (CP) complex. Knockdown of the CP β subunit increased erythroid maturation in ex vivo cultures and decreased colony forming potential of stress erythroid precursors. In a genetic complementation assay for Samd14 activity, our results revealed that the Samd14-CP interaction is a determinant of erythroid precursor cell levels and function. Samd14-CP promotes SCF/c-kit signaling in CD71med spleen erythroid precursors.
HostingRepository	PRIDE
AnnounceDate	2022-08-09
AnnouncementXML	Submission_2022-08-09_07:51:03.167.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030467
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sophe Alvarez
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-16 12:33:55	ID requested
⏵ 1	2022-08-09 07:51:03	announced

Dataset with its publication pending

submitter keyword: Samd14

capping protein

c-Kit

erythropoietin

stress erythropoiesis

Sophie Alvarez
contact affiliation	Proteomics and Metabolomics Facility, University of Nebraska- Lincoln
contact email	salvarez@unl.edu
lab head
Sophe Alvarez
contact affiliation	University of Nebraska - Lincoln
contact email	salvarez@unl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD030467

PRIDE project URI

• PRIDE
  1. PXD030467
     1. Label: PRIDE project
     2. Name: Functional Requirements for a Samd14-Capping Protein Complex in Stress Erythropoiesis