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PXD030466-1

PXD030466 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleWriting and erasing O-GlcNAc on casein kinase 2 alpha alters the phosphoproteome
DescriptionO-GlcNAc is an essential carbohydrate modification that intersects with phosphorylation signaling pathways via crosstalk on protein substrates or by direct modification of the kinases that write the phosphate modification. Casein kinase 2 alpha (CK2), the catalytic subunit of the ubiquitously expressed and constitutively active kinase CK2, is modified by O-GlcNAc, but the effect of this modification on the phosphoproteome in cells is unknown. Here, we apply complementary targeted O-GlcNAc editors, nanobody-OGT and -splitOGA, to selectively write and erase O-GlcNAc from a tagged CK2 to measure the effects on the phosphoproteome in cells. These tools effectively and selectively edit the S347 glycosite on CK2. Using quantitative phosphoproteomics, we report 51 proteins whose enrichment changes as a function of editing O-GlcNAc on CK2, including HDAC1, HDAC2, ENSA, SMARCAD1, and PABPN1. Specific phosphosites on HDAC1 S393 and HDAC2 S394, both reported CK2 substrates, are significantly enhanced by O-GlcNAcylation of CK2. These data will propel future studies on the crosstalk between O-GlcNAc and phosphorylation.
HostingRepositoryPRIDE
AnnounceDate2022-04-26
AnnouncementXMLSubmission_2022-04-25_19:12:54.423.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristina Woo
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-16 12:32:35ID requested
12022-04-25 19:12:54announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: O-GlcNAc, phosphoproteomics, casein kinase 2, CK2
Contact List
Christina May Woo
contact affiliationAssociate Professor of Chemistry and Chemical Biology Department of Chemistry and Chemical Biology Harvard University, USA
contact emailcwoo@chemistry.harvard.edu
lab head
Christina Woo
contact affiliationHarvard University
contact emailcwoo@chemistry.harvard.edu
dataset submitter
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