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PXD030429-1

PXD030429 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMiniTurbo-based interactomics of two plasma membrane-localized SNARE proteins in Marchantia polymorpha
Description• Marchantia polymorpha is a model liverwort and its overall low genetic redundancy is advantageous for dissecting complex pathways. Proximity-dependent in vivo biotin-labelling methods have emerged as powerful interactomics tools in recent years. However, interactomics studies applying proximity labelling are currently limited to angiosperm species in plants. • Here, we established and evaluated a miniTurbo-based interactomics method in M. polymorpha using MpSYP12A and MpSYP13B, two plasma membrane-localized SNARE proteins, as baits. • We show that our method yields a manifold of potential interactors of MpSYP12A and MpSYP13B compared to a co-immunoprecipitation approach. Our method could capture specific candidates for each SNARE. • We conclude that a miniTurbo-based method is a feasible tool for interactomics in M. polymorpha, potentially applicable to other model bryophytes. Our interactome dataset on MpSYP12A and MpSYP13B will be a useful resource to elucidate the evolution of SNARE functions.
HostingRepositoryPRIDE
AnnounceDate2022-04-01
AnnouncementXMLSubmission_2022-04-01_04:51:17.501.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSara Christina Stolze
SpeciesList scientific name: Marchantia polymorpha; NCBI TaxID: 3197;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-15 09:27:06ID requested
12022-04-01 04:51:17announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: interactomics, Marchantia polymorpha, membrane-trafficking, miniTurbo, proximity labelling, SNARE protein
Contact List
Hirofumi Nakagami
contact affiliationBasic Immune System of Plants / Protein Mass Spectrometry Max Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany
contact emailnakagami@mpipz.mpg.de
lab head
Sara Christina Stolze
contact affiliationPlant Proteomics and Mass Spectrometry Group Max Planck Institute for Plant Breeding Research Carl-von-Linné Weg 10 50829 Cologne
contact emailstolze@mpipz.mpg.de
dataset submitter
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Dataset FTP location
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