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PXD030266-1

PXD030266 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDepletion of the RNA-binding protein PURA triggers changes in posttranscriptional gene regulation and loss of P-bodies
DescriptionThe RNA-binding protein PURA has been implicated in the rare, monogenetic, neurodevelopmental disorder PURA Syndrome. PURA binds both DNA and RNA and has been associated with various cellular functions. Only little is known about its main cellular functions and the molecular pathways affected upon PURA depletion. Here, we show that PURA is predominantly located in the cytoplasm, where it binds to thousands of mRNAs. Many of these transcripts change abundance in response to PURA depletion. The encoded proteins suggest a role for PURA in immune responses, mitochondrial function, autophagy and processing (P)-body activity. Intriguingly, reduced PURA levels decrease the expression of the integral P-body components LSM14A and DDX6, and strongly affect P-body formation in human cells. Furthermore, PURA knockdown results in stabilization of P-body-enriched transcripts, whereas other mRNAs decrease. Hence, reduced PURA levels, as reported in patients with PURA Syndrome, influence the formation and composition of this phase-separated RNA processing machinery. Our study on PURA provides a blueprint for the comprehensive understanding of the tight connection between P-body-associated RNA regulation and neurodevelopmental disorders.
HostingRepositoryPRIDE
AnnounceDate2022-12-19
AnnouncementXMLSubmission_2022-12-19_02:38:15.495.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJulianeMerl-Pham
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-08 12:53:56ID requested
12022-12-19 02:38:15announced
22023-11-14 08:59:12announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: PurA
Contact List
DierkNiessing
contact affiliationInstitute of Structural Biology, Helmholtz Zentrum München - German Research Center for Environmental Health, 85764, Neuherberg, Germany; Institute of Pharmaceutical Biotechnology, 89081, Ulm University, Ulm, Germany.
contact emailniessing@helmholtz-muenchen.de
lab head
JulianeMerl-Pham
contact affiliationResearch Unit Protein Science, Helmholtz Center Munich
contact emailjuliane.merl@helmholtz-muenchen.de
dataset submitter
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