<<< Full experiment listing


PXD030111 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSuppressors of amyloid-beta toxicity improve recombinant protein production by reducing oxidative stress and tunning cellular metabolism
DescriptionIn this study, we used a yeast Alzheimer’s disease model in which amyloid-β peptides (Aβ42) accumulate and induce stress-related phenotypes similar to overexpression of recombinant proteins. We validated that suppressors of Aβ42 cytotoxicity could reduce cell stress and improve recombinant protein production. Omics analyses and reverse metabolic engineering reveal potential regulatory hubs in cellular metabolism and protein synthesis, which may provide guidelines for engineering other cell factories for efficient protein production.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEgor Vorontsov
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-02 03:18:49ID requested
12022-05-12 09:16:00announced
Publication List
Chen X, Li X, Ji B, Wang Y, Ishchuk OP, Vorontsov E, Petranovic D, Siewers V, Engqvist MKM, Suppressors of amyloid-β toxicity improve recombinant protein production in yeast by reducing oxidative stress and tuning cellular metabolism. Metab Eng, 72():311-324(2022) [pubmed]
Keyword List
submitter keyword: Alzheimer’s disease, amyloid-beta, yeast, Saccharomyces cerevisiae, tandem mass tag, TMTpro, Orbitrap Fusion Lumos
Contact List
Martin Engqvist
contact affiliationDivision of Systems and Synthetic Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden
contact emailmartin.engqvist@chalmers.se
lab head
Egor Vorontsov
contact affiliationProteomics Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
contact emailyegor.msu@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD030111
PRIDE project URI
Repository Record List
[ + ]