PXD030111 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Suppressors of amyloid-beta toxicity improve recombinant protein production by reducing oxidative stress and tunning cellular metabolism |
Description | In this study, we used a yeast Alzheimer’s disease model in which amyloid-β peptides (Aβ42) accumulate and induce stress-related phenotypes similar to overexpression of recombinant proteins. We validated that suppressors of Aβ42 cytotoxicity could reduce cell stress and improve recombinant protein production. Omics analyses and reverse metabolic engineering reveal potential regulatory hubs in cellular metabolism and protein synthesis, which may provide guidelines for engineering other cell factories for efficient protein production. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-12 |
AnnouncementXML | Submission_2022-05-12_09:15:59.734.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Egor Vorontsov |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-12-02 03:18:49 | ID requested | |
⏵ 1 | 2022-05-12 09:16:00 | announced | |
2 | 2023-11-14 08:54:25 | announced | 2023-11-14: Updated project metadata. |
Publication List
Chen X, Li X, Ji B, Wang Y, Ishchuk OP, Vorontsov E, Petranovic D, Siewers V, Engqvist MKM, toxicity improve recombinant protein production in yeast by reducing oxidative stress and tuning cellular metabolism. Metab Eng, 72():311-324(2022) [pubmed] |
10.1016/J.YMBEN.2022.04.005; |
Keyword List
submitter keyword: Alzheimer’s disease, amyloid-beta, yeast, Saccharomyces cerevisiae, tandem mass tag, TMTpro, Orbitrap Fusion Lumos |
Contact List
Martin Engqvist |
contact affiliation | Division of Systems and Synthetic Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden |
contact email | martin.engqvist@chalmers.se |
lab head | |
Egor Vorontsov |
contact affiliation | Proteomics Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden |
contact email | yegor.msu@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030111
- Label: PRIDE project
- Name: Suppressors of amyloid-beta toxicity improve recombinant protein production by reducing oxidative stress and tunning cellular metabolism