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PXD030111

PXD030111 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSuppressors of amyloid-beta toxicity improve recombinant protein production by reducing oxidative stress and tunning cellular metabolism
DescriptionIn this study, we used a yeast Alzheimer’s disease model in which amyloid-β peptides (Aβ42) accumulate and induce stress-related phenotypes similar to overexpression of recombinant proteins. We validated that suppressors of Aβ42 cytotoxicity could reduce cell stress and improve recombinant protein production. Omics analyses and reverse metabolic engineering reveal potential regulatory hubs in cellular metabolism and protein synthesis, which may provide guidelines for engineering other cell factories for efficient protein production.
HostingRepositoryPRIDE
AnnounceDate2022-05-12
AnnouncementXMLSubmission_2022-05-12_09:15:59.734.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEgor Vorontsov
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-02 03:18:49ID requested
12022-05-12 09:16:00announced
Publication List
Chen X, Li X, Ji B, Wang Y, Ishchuk OP, Vorontsov E, Petranovic D, Siewers V, Engqvist MKM, Suppressors of amyloid-β toxicity improve recombinant protein production in yeast by reducing oxidative stress and tuning cellular metabolism. Metab Eng, 72():311-324(2022) [pubmed]
10.1016/J.YMBEN.2022.04.005;
Keyword List
submitter keyword: Alzheimer’s disease, amyloid-beta, yeast, Saccharomyces cerevisiae, tandem mass tag, TMTpro, Orbitrap Fusion Lumos
Contact List
Martin Engqvist
contact affiliationDivision of Systems and Synthetic Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden
contact emailmartin.engqvist@chalmers.se
lab head
Egor Vorontsov
contact affiliationProteomics Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
contact emailyegor.msu@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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