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PXD030092-1

PXD030092 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe proteome of irradiated glioma stem cells and of their extracellular vesicles
DescriptionGlioblastoma is a malignant brain tumor that is highly resistant to radiation and chemotherapy, where patients survive on average only 15 months after diagnosis. Furthering the understanding of mechanisms leading to radiation resistance of glioma is paramount to identify novel therapeutic targets. Previous studies have shown that glioma stem cells (GSCs) play an important role in promoting radiation resistance and disease recurrence. Herein we analyze the proteomic alterations occurring in patient-derived GSCs upon radiation treatment in order to identify molecular drivers of resistance. We show that proteome changes upon radiation accurately predict the resistance status of the cells, and that resistance to radiation does not correlate with glioma transcriptional subtypes. We further show that the radio-resistant cell line GSC-267 sheds microvesicles (MVs) enriched in the metabolic enzyme nicotinamide phosphoribosyltransferase (NAMPT).
HostingRepositoryPRIDE
AnnounceDate2023-04-06
AnnouncementXMLSubmission_2023-04-06_14:44:21.212.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterElenaPanizza
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-02 02:05:00ID requested
12023-04-06 14:44:21announced
22023-11-14 08:53:56announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: glioblastoma, glioma, radiation, glioma stem cells,human
Contact List
Richard A.Cerione
contact affiliationDepartment of Molecular Medicine, Cornell University, Ithaca, NY 14853 Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853
contact emailrac1@cornell.edu
lab head
ElenaPanizza
contact affiliationCornell University
contact emailelena.panizza84@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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