PXD029802-1
PXD029802 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome profiling of PIP4K depleted cells |
Description | Phosphatidylinositol-5-phosphate 4-kinases (PIP4Ks) family include PIP4K2A, PIP4K2B and PIP4K2C isoforms that phosphorylate phosphatidylinositol-5-phosphate on fourth position of inositol ring and catalyse PtdIns(4,5)P2 formation. These enzymes are central to cellular processes such as growth and development, metabolism, and tumour growth. A major limitation towards understanding of how these kinases promote systemic cellular responses and safeguard genomic integrity, is posed by the absence of comprehensive characterization of their downstream target proteins. In order to identify the potential effectors playing downstream of these kinases, we depleted PIP4K2A, PIP4K2B or both in human osteosarcoma cells (U2OS) followed by largescale proteomics analysis. Using LC-MS/MS approach, we report that the downregulation PIP4K2A, PIP4K2B or both, is correlated with mis-regulation of proteins that are involved in cellular processes impairing genome instability. The analysis further revealed impact on a cluster of proteins (including post translational modifications) involved in genome integrity maintenance. |
HostingRepository | jPOST |
AnnounceDate | 2022-11-19 |
AnnouncementXML | Submission_2022-11-18_07:00:11.949.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Poorwa Awasthi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-11-18 21:12:38 | ID requested | |
⏵ 1 | 2022-11-18 07:00:12 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: PIP4Ks, cancer, DNA repair, genome instability |
Contact List
Amit Kumar | |
---|---|
lab head | |
Poorwa Awasthi | |
contact affiliation | CSIR-Indian Institute of Toxicology Research |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001387/ |