PXD029802

PXD029802 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteome profiling of PIP4K depleted cells
Description	Phosphatidylinositol-5-phosphate 4-kinases (PIP4Ks) family include PIP4K2A, PIP4K2B and PIP4K2C isoforms that phosphorylate phosphatidylinositol-5-phosphate on fourth position of inositol ring and catalyse PtdIns(4,5)P2 formation. These enzymes are central to cellular processes such as growth and development, metabolism, and tumour growth. A major limitation towards understanding of how these kinases promote systemic cellular responses and safeguard genomic integrity, is posed by the absence of comprehensive characterization of their downstream target proteins. In order to identify the potential effectors playing downstream of these kinases, we depleted PIP4K2A, PIP4K2B or both in human osteosarcoma cells (U2OS) followed by largescale proteomics analysis. Using LC-MS/MS approach, we report that the downregulation PIP4K2A, PIP4K2B or both, is correlated with mis-regulation of proteins that are involved in cellular processes impairing genome instability. The analysis further revealed impact on a cluster of proteins (including post translational modifications) involved in genome integrity maintenance.
HostingRepository	jPOST
AnnounceDate	2022-11-19
AnnouncementXML	Submission_2022-11-18_07:00:11.949.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Poorwa Awasthi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-18 21:12:38	ID requested
⏵ 1	2022-11-18 07:00:12	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: PIP4Ks, cancer, DNA repair, genome instability

submitter keyword: PIP4Ks, cancer, DNA repair, genome instability

Contact List

Amit Kumar
lab head
Poorwa Awasthi
contact affiliation	CSIR-Indian Institute of Toxicology Research
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001387/