PXD029763

PXD029763 is an original dataset announced via ProteomeXchange.

Title	Differential membrane proteomics of PEDV infected Vero cells
Description	PEDV wildtype strain (HNXX) infected Vero cells could form typical syncytial lesions, resulting in cell membrane rearrangement. In order to study which proteins are involved in the rearrangement of cell membrane, we infected Vero cells with PEDV. To capture the intact cell membrane at the same time as the occurrence of lesions caused by PEDV, membrane protein was collected at 18 hours post infection. iTRAQ-based quantitative membrane proteomics was used in analysing host proteins differentially expressed in PEDV-infected Vero cells and the control group. In this quantitative proteomics experiments results, a total of 4579 proteins were identified. Significantly upregulated or downregulated proteins were determined with a p-value of <0.05 and FC of <0.83 or >1.20. After bioinformatics data analysis, Ten proteins were found significantly upregulated, and eleven proteins were markedly downregulated after virus infection. Approximately 47% of these proteins were membrane proteins or membrane-associated proteins.
HostingRepository	iProX
AnnounceDate	2021-11-14
AnnouncementXML	Submission_2022-10-19_20:31:42.634.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huimin Huang
SpeciesList	scientific name: Porcine epidemic diarrhea virus; NCBI TaxID: 28295;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-15 23:52:53	ID requested
⏵ 1	2022-10-19 20:31:43	announced

Huang H, Li Y, Wang L, Song Y, Zhang G, Membrane proteomic analysis identifies the polarity protein PARD3 as a novel antiviral protein against PEDV infection. J Proteomics, 253():104462(2022) [pubmed]

submitter keyword: membrane, proteomics, PEDV, Vero cells

Gaiping Zhang
contact affiliation	Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences
contact email	zhanggaip@126.com
lab head
Huimin Huang
contact affiliation	College of Veterinary Medicine, Henan Agricultural University
contact email	623122533@qq.com
dataset submitter

iProX dataset URI