PXD029705 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Immunoprecipitation of p97 from X.laevis S-phase chromatin |
Description | Complex cellular processes are driven by the regulated assembly and disassembly of large multi-protein complexes. In eukaryotic DNA replication, whilst we are beginning to understand the molecular mechanism for assembly of the replication machinery (replisome), we still know little about the regulation of its disassembly at replication termination. Over recent years, the first elements of this process emerged: the replicative helicase at the heart of the replisome is polyubiquitylated prior to unloading and that this unloading requires p97 segregase activity. Two different E3 ubiquitin ligases are now known to ubiquitylate the helicase under different conditions: Cul2Lrr1 and TRAIP. To understand how p97 is targeted to the terminated replisome, we immunoprecipitated p97 from chromatin replicating in Xenopus laevis egg extract. We have found a p97 cofactor, Ubxn7, which can interact with active Cul2Lrr1 and with p97, facilitating efficient recognition and processing of terminated helicases ubiquitylated by Cul2. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:32:52.540.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD029705 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Aga Gambus |
SpeciesList | scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-11-12 06:06:39 | ID requested | |
1 | 2022-10-14 02:18:35 | announced | |
⏵ 2 | 2023-11-14 08:32:55 | announced | 2023-11-14: Updated project metadata. |
Publication List
10.6019/PXD029705; |
Tarcan Z, Poovathumkadavil D, Skagia A, Gambus A, The p97 segregase cofactor Ubxn7 facilitates replisome disassembly during S-phase. J Biol Chem, 298(8):102234(2022) [pubmed] |
Keyword List
submitter keyword: VCP, replisome disassembly, Xenopus laevis, p97, DNA replication |
Contact List
Aga Gambus |
contact affiliation | Chromosomal Replication Laboratory, Institute for Cancer and Genomic Sciences, IBR west, WX2.67, University of Birmingham, Edgbaston, Birmingham, B15 2TT |
contact email | a.gambus@bham.ac.uk |
lab head | |
Aga Gambus |
contact affiliation | University of Birmingham |
contact email | a.gambus@bham.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029705
- Label: PRIDE project
- Name: Immunoprecipitation of p97 from X.laevis S-phase chromatin