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PXD029705

PXD029705 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleImmunoprecipitation of p97 from X.laevis S-phase chromatin
DescriptionComplex cellular processes are driven by the regulated assembly and disassembly of large multi-protein complexes. In eukaryotic DNA replication, whilst we are beginning to understand the molecular mechanism for assembly of the replication machinery (replisome), we still know little about the regulation of its disassembly at replication termination. Over recent years, the first elements of this process emerged: the replicative helicase at the heart of the replisome is polyubiquitylated prior to unloading and that this unloading requires p97 segregase activity. Two different E3 ubiquitin ligases are now known to ubiquitylate the helicase under different conditions: Cul2Lrr1 and TRAIP. To understand how p97 is targeted to the terminated replisome, we immunoprecipitated p97 from chromatin replicating in Xenopus laevis egg extract. We have found a p97 cofactor, Ubxn7, which can interact with active Cul2Lrr1 and with p97, facilitating efficient recognition and processing of terminated helicases ubiquitylated by Cul2.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:32:52.540.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD029705
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAga Gambus
SpeciesList scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationListphosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-12 06:06:39ID requested
12022-10-14 02:18:35announced
22023-11-14 08:32:55announced2023-11-14: Updated project metadata.
Publication List
10.6019/PXD029705;
Keyword List
submitter keyword: VCP, replisome disassembly, Xenopus laevis, p97, DNA replication
Contact List
Aga Gambus
contact affiliationChromosomal Replication Laboratory, Institute for Cancer and Genomic Sciences, IBR west, WX2.67, University of Birmingham, Edgbaston, Birmingham, B15 2TT
contact emaila.gambus@bham.ac.uk
lab head
Aga Gambus
contact affiliationUniversity of Birmingham
contact emaila.gambus@bham.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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