PXD029634

PXD029634 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of peripheral blood mononuclear cells isolated from patients with pulmonary tuberculosis: a pilot study from Zanzibar, Tanzania
Description	This study aimed at exploring the proteomic profile of PBMCs to predict response to treatment in pulmonary tuberculosis (PTB). This was a pilot study conducted among 8 adult patients from Zanzibar, Tanzania with confirmed PTB. Blood samples were collected at baseline, at 2 months of treatment, and at the end of 6 months of treatment. Proteins were extracted from PBMCs and analysed using LC‑MS/MS‑based label‑free quantitative proteomics. Overall, 3,530 proteins were quantified across the samples, and 12 differentially expressed proteins were identified at both 2 months of treatment and at treatment completion, which were involved in cellular and metabolic processes, as well as binding and catalytic activity. Seven were downregulated proteins (HSPA1B/HSPA1A, HSPH1, HSP90AA1, lipopolysaccharide binding protein, complement component 9, calcyclin-binding protein, and protein transport protein Sec31A), and 5 proteins were upregulated (SEC14 domain and spectrin repeat-containing protein 1, leucine-rich repeat-containing 8 VRAC subunit D, homogentisate 1,2-dioxygenase, NEDD8-activating enzyme E1 regulatory subunit, and N-acetylserotonin O-methyltransferase-like protein). The results showed that proteome analysis of PBMCs can be used as a novel technique to identify potential biomarkers to assess treatment efficacy in PTB. The novel proteins elucidated in this work may provide new insights for understanding PTB pathogenesis, treatment, and prognosis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:37:22.472.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Even Birkeland
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-09 06:07:54	ID requested
1	2023-03-10 21:56:47	announced
⏵ 2	2023-11-14 08:37:24	announced	2023-11-14: Updated project metadata.

Barakat A, Birkeland E, J, ø, rstad MD, El Hajj M, Marijani M, D, ø, skeland A, Mjaavatten O, Berven FS, Mustafa T, Proteomic analysis of peripheral blood mononuclear cells isolated from patients with pulmonary tuberculosis: A pilot study from Zanzibar, Tanzania. PLoS One, 18(2):e0281757(2023) [pubmed]

submitter keyword: Biomarkers

Peripheral blood mononuclear cells

Proteomics

Pulmonary tuberculosis

Treatment response.

Tehmina Mustafa
contact affiliation	Centre of International Health, Department of Global Public Health and Primary Care, University of Bergen, Norway
contact email	Tehmina.Mustafa@uib.no
lab head
Even Birkeland
contact affiliation	Institute of biomedicine, University of BErgen
contact email	even.birkeland@uib.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD029634

PRIDE project URI

• PRIDE
  1. PXD029634
     1. Label: PRIDE project
     2. Name: Proteomic analysis of peripheral blood mononuclear cells isolated from patients with pulmonary tuberculosis: a pilot study from Zanzibar, Tanzania