PXD029431-1

PXD029431 is an original dataset announced via ProteomeXchange.

Title	Enrichment and Proteomic Identification of Cryptosporidium parvum Oocyst Wall
Description	The Cryptosporidium parvum (C. parvum) oocyst wall provides strong protection against hostile environmental factors; however, research is limited concerning about the oocyst wall at the proteomic level. In this study, a comprehensive analysis of the proteome expressed by the oocyst wall of C. parvum was performed using label-free qualitative high-performance liquid chromatography (HPLC) fractionation and mass spectrometry-based qualitative proteomics technologies. A total of 798 proteins were identified, accounting for about 20% of the CryptoDB proteome. By using bioinformatic analysis, functional annotation and subcellular localization of the identified proteins were examined for better understanding of the characteristics of the oocyst wall. Among the identified proteins, one protein encoded by the C. parvum cgd7_5140 (Cpcgd7_5140) gene was predicted to be located on the surface of the oocyst wall. To verify its localization, an indirect immunofluorescent antibody assay (IFA) demonstrated that the Cpcgd7_5140 was localized on the surface of the oocyst wall, illustrating the potential usage as a marker for C. parvum detection in vitro. The results provide new information about the proteomic composition of the Cryptosporidium oocyst wall, thereby providing a theoretical basis for further study of Cryptosporidium oocyst wall formation as well as the selection of targets for Cryptosporidium detection.
HostingRepository	PRIDE
AnnounceDate	2022-10-05
AnnouncementXML	Submission_2022-10-05_00:27:15.843.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wang Luyang
SpeciesList	scientific name: Cryptosporidium parvum; NCBI TaxID: 5807;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-29 04:26:32	ID requested
⏵ 1	2022-10-05 00:27:16	announced
2	2023-11-14 08:54:40	announced	2023-11-14: Updated project metadata.

10.1186/S13071-022-05448-8;

submitter keyword: Cryptosporidium parvum

Oocyst wall

Proteomics

Detection

Longxian Zhang
contact affiliation	College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
contact email	zhanglx8999@henau.edu.cn
lab head
Wang Luyang
contact affiliation	College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
contact email	wly952659965@foxmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD029431

PRIDE project URI

• PRIDE
  1. PXD029431
     1. Label: PRIDE project
     2. Name: Enrichment and Proteomic Identification of Cryptosporidium parvum Oocyst Wall