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PXD029431

PXD029431 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEnrichment and Proteomic Identification of Cryptosporidium parvum Oocyst Wall
DescriptionThe Cryptosporidium parvum (C. parvum) oocyst wall provides strong protection against hostile environmental factors; however, research is limited concerning about the oocyst wall at the proteomic level. In this study, a comprehensive analysis of the proteome expressed by the oocyst wall of C. parvum was performed using label-free qualitative high-performance liquid chromatography (HPLC) fractionation and mass spectrometry-based qualitative proteomics technologies. A total of 798 proteins were identified, accounting for about 20% of the CryptoDB proteome. By using bioinformatic analysis, functional annotation and subcellular localization of the identified proteins were examined for better understanding of the characteristics of the oocyst wall. Among the identified proteins, one protein encoded by the C. parvum cgd7_5140 (Cpcgd7_5140) gene was predicted to be located on the surface of the oocyst wall. To verify its localization, an indirect immunofluorescent antibody assay (IFA) demonstrated that the Cpcgd7_5140 was localized on the surface of the oocyst wall, illustrating the potential usage as a marker for C. parvum detection in vitro. The results provide new information about the proteomic composition of the Cryptosporidium oocyst wall, thereby providing a theoretical basis for further study of Cryptosporidium oocyst wall formation as well as the selection of targets for Cryptosporidium detection.
HostingRepositoryPRIDE
AnnounceDate2022-10-05
AnnouncementXMLSubmission_2022-10-05_00:27:15.843.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWang Luyang
SpeciesList scientific name: Cryptosporidium parvum; NCBI TaxID: 5807;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-29 04:26:32ID requested
12022-10-05 00:27:16announced
Publication List
10.1186/S13071-022-05448-8;
Keyword List
submitter keyword: Cryptosporidium parvum
Oocyst wall
Proteomics
Detection
Contact List
Longxian Zhang
contact affiliationCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
contact emailzhanglx8999@henau.edu.cn
lab head
Wang Luyang
contact affiliationCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
contact emailwly952659965@foxmail.com
dataset submitter
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Dataset FTP location
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