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PXD029396-1

PXD029396 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleActinobacillus pleuropneumoniae serotype 2 exoproteome LF-LC-MS/MS
DescriptionPorcine pleuropneumonia caused by Actinobacillus pleuropneumoniae affects pig health status and the swine industry worldwide. Despite of the extensive number of studies focused on A. pleuropneumoniae infection and vaccine development, its exoproteome is still rather unexplored. By combining high-throughput mass spectrometry and immunoproteomic approaches, with our current work we provide an in-depth characterisation of A. pleuropneumoniae serotype 2 exoproteome. Label-free liquid chromatography - tandem mass spectrometry (LC-MS/MS) combined with a comprehensive bioinformatics analysis revealed 484 secreted proteins, of which 84 were predicted to be virulence factors and 142 to be exported via different export mechanisms. The RTX toxins ApxIIA, ApxIIIA and ApxIVA were found to be the most abundant proteins in the A. pleuropneumoniae serotype 2 exoproteome, although ApxIVA is commonly assumed to be expressed exclusively in vivo. Immunoproteomic approaches coupled to LC-MS/MS analysis allowed to portray the immunogenic proteins within the bacterial exoproteome to identify potential vaccine candidates. Using serum pools from uninfected, acutely infected and chronically infected animals, we were able to monitor the seroconversion during disease progression. Overall, our work is expected to contribute to the understanding of the complex pathogenic mechanisms and to facilitate the discovery of potential antimicrobial agents for controlling porcine pleuropneumonia.
HostingRepositoryPRIDE
AnnounceDate2022-10-13
AnnouncementXMLSubmission_2022-10-13_07:07:46.833.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStelliStancheva
SpeciesList scientific name: Actinobacillus pleuropneumoniae serovar 2; NCBI TaxID: 34063;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-28 06:41:30ID requested
12022-10-13 07:07:47announced
22023-11-14 08:54:29announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: porcine pleuropneumonia, exoproteome,Actinobacillus pleuropneumoniae
Contact List
MonikaEhling-Schulz
contact affiliationInstitute of Microbiology Department of Pathobiology University of Veterinary Medicine Veterinaerplatz 1 1210 Vienna Austria
contact emailMonika.Ehling-Schulz@vetmeduni.ac.at
lab head
StelliStancheva
contact affiliationUniversity of Veterinary Medicine, Vienna, Austria
contact emailstelli.stancheva@vetmeduni.ac.at
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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