PXD029325-2

PXD029325 is an original dataset announced via ProteomeXchange.

Title	Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system
Description	Long-distance regeneration of the central nervous system (CNS) has been achieved from the eye to the brain through activation of neuronal molecular pathways or pharmacological approaches. Characterizing the mature neuronal environment is essential to understand the adult axonal guidance in order to complete the circuit reconstruction. To this end, we used mass spectrometry (MS)-based quantitative proteomics to characterize the proteomes of major nuclei of the adult visual system: suprachiasmatic nucleus (SCN), ventral and dorsal lateral geniculate nucleus (vLGN, dLGN) and superior colliculus (SCol)), as well as the optic chiasm. These analyses revealed the presence of guidance molecules and guidance-associated factors in the adult visual targets. Moreover, by performing bilateral optic nerve crush, we showed that the expression of specific proteins was significantly modulated by the injury in the visual targets, even in the ones most distal to the lesion site. On another hand, we found that the expression of guidance molecules was not modified upon injury and that regenerative axons are able to respond to guidance cues. Together, our results provide an extensive characterization of the molecular environment in intact and injured conditions.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:00:33.381.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029325
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hesse Anne-Marie
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-25 02:42:12	ID requested
1	2022-10-25 02:41:49	announced
⏵ 2	2023-11-14 08:00:36	announced	2023-11-14: Updated project metadata.

Vilallongue N, Schaeffer J, Hesse AM, Delpech C, Blot B, Paccard A, Plissonnier E, Excoffier B, Cout, é Y, Belin S, Nawabi H, Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system. Nat Commun, 13(1):6040(2022) [pubmed]

submitter keyword: LC-MS/MS, QExactive HF, CNS, mouse

Yohann Coute
contact affiliation	Univ. Grenoble Alpes, INSERM, CEA, UMR BioSanté U1292, CNRS, CEA, FR2048 38000, Grenoble, France
contact email	yohann.coute@cea.fr
lab head
Hesse Anne-Marie
contact affiliation	Laboratoire d'Etude de la Dynamique des Protéomes
contact email	anne-marie.hesse@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029325
     1. Label: PRIDE project
     2. Name: Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system