PXD029289

PXD029289 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometric identification of antimicrobial peptides from medicinal seeds
Description	Traditional medicinal plants contain a variety of bioactive natural products including cysteine-rich (Cys-rich) antimicrobial peptides (AMPs). Cys-rich AMPs are often crosslinked by multiple disulfide bonds which increase their resistance to chemical and enzymatic degradation. However, this class of molecules is relatively underexplored. Herein, in silico analysis predicted 80 – 100 Cys-rich AMPs per species from three edible traditional medicinal plants: Linum usitatissimum (flax), Trifolium pratense (red clover), and Sesamum indicum (sesame). Proteomics analysis of seed peptide extracts revealed direct evidence for the translation of 3-10 Cys-rich AMPs per species including lipid transfer proteins, defensins, α-hairpinins, and snakins. Negative activity revealed by antibacterial screening highlights the importance of employing a multi-pronged approach for AMP discovery. Further, this study demonstrates that flax, red clover, and sesame are promising sources of further AMP discovery and characterization.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_09:40:36.355.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029289
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Leslie Hicks
SpeciesList	scientific name: Linum usitatissimum; NCBI TaxID: 4006; scientific name: Sesamum indicum; NCBI TaxID: 4182; scientific name: Trifolium pratense; NCBI TaxID: 57577;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-22 06:57:13	ID requested
⏵ 1	2022-02-16 09:40:37	announced

Moyer TB, Brechbill AM, Hicks LM, Mass Spectrometric Identification of Antimicrobial Peptides from Medicinal Seeds. Molecules, 26(23):(2021) [pubmed]

submitter keyword: Antimicrobial peptides, Cysteine-rich peptides, Linum usitatissimum, flax, Trifolium pretense, red clover, Sesamum indicum, sesame

Leslie M. Hicks
contact affiliation	University of North Carolina at Chapel Hill, Department of Chemistry
contact email	lmhicks@unc.edu
lab head
Leslie Hicks
contact affiliation	University of North Carolina at Chapel Hill
contact email	lmhicks@unc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029289
     1. Label: PRIDE project
     2. Name: Mass spectrometric identification of antimicrobial peptides from medicinal seeds