PXD029289 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometric identification of antimicrobial peptides from medicinal seeds |
Description | Traditional medicinal plants contain a variety of bioactive natural products including cysteine-rich (Cys-rich) antimicrobial peptides (AMPs). Cys-rich AMPs are often crosslinked by multiple disulfide bonds which increase their resistance to chemical and enzymatic degradation. However, this class of molecules is relatively underexplored. Herein, in silico analysis predicted 80 – 100 Cys-rich AMPs per species from three edible traditional medicinal plants: Linum usitatissimum (flax), Trifolium pratense (red clover), and Sesamum indicum (sesame). Proteomics analysis of seed peptide extracts revealed direct evidence for the translation of 3-10 Cys-rich AMPs per species including lipid transfer proteins, defensins, α-hairpinins, and snakins. Negative activity revealed by antibacterial screening highlights the importance of employing a multi-pronged approach for AMP discovery. Further, this study demonstrates that flax, red clover, and sesame are promising sources of further AMP discovery and characterization. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-16 |
AnnouncementXML | Submission_2022-02-16_09:40:36.355.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD029289 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Leslie Hicks |
SpeciesList | scientific name: Linum usitatissimum; NCBI TaxID: 4006; scientific name: Sesamum indicum; NCBI TaxID: 4182; scientific name: Trifolium pratense; NCBI TaxID: 57577; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-10-22 06:57:13 | ID requested | |
⏵ 1 | 2022-02-16 09:40:37 | announced | |
Publication List
Moyer TB, Brechbill AM, Hicks LM, Mass Spectrometric Identification of Antimicrobial Peptides from Medicinal Seeds. Molecules, 26(23):(2021) [pubmed] |
Keyword List
submitter keyword: Antimicrobial peptides, Cysteine-rich peptides, Linum usitatissimum, flax, Trifolium pretense, red clover, Sesamum indicum, sesame |
Contact List
Leslie M. Hicks |
contact affiliation | University of North Carolina at Chapel Hill, Department of Chemistry |
contact email | lmhicks@unc.edu |
lab head | |
Leslie Hicks |
contact affiliation | University of North Carolina at Chapel Hill |
contact email | lmhicks@unc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029289
- Label: PRIDE project
- Name: Mass spectrometric identification of antimicrobial peptides from medicinal seeds