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PXD029270-1

PXD029270 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCysteine 253 of UCP1 regulates energy expenditure and adipose tissue inflammation
DescriptionUncoupling protein 1 (UCP1) is thought to be a major regulator of whole-body energy expenditure and metabolic homeostasis. However, the widely employed UCP1 loss of function model has recently been shown to have destructive effects on the entire electron transport chain of thermogenic fat. As such, the role of UCP1 in metabolic regulation in vivo remains unclear. We recently identified cysteine-253 as an allosteric site on UCP1 that elevates protein activity upon covalent modification. Here we examine the physiological importance of this site through the generation of a UCP1 cysteine-253 null mouse (UCP1 C253A), the first genetic model for selective disruption of UCP1 in vivo. UCP1 C253A mice exhibit significantly compromised thermogenic responses but display no measurable effect on fat accumulation in an obesogenic environment. Unexpectedly, instead we find that lack of cysteine-253 results in substantial immune cell infiltration and inflammatory pathology in adipose tissues of male, but not female mice. Together, our results establish the UCP1 cysteine-253 activation site as a regulator of acute thermogenesis and sex-dependent adipose tissue inflammation.
HostingRepositoryPRIDE
AnnounceDate2021-12-15
AnnouncementXMLSubmission_2021-12-15_03:06:33.374.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHaopeng Xiao
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue
InstrumentOrbitrap Eclipse; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-21 05:53:03ID requested
12021-12-15 03:06:33announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Thermogenesis, UCP1, inflammation, adipose, sex-dependent
Contact List
Edward Chouchani
contact affiliation1 Department of Cancer Biology, Dana–Farber Cancer Institute, Boston, MA, USA. 2 Department of Cell Biology, Harvard Medical School, Boston, MA, USA.
contact emailEdwardT_Chouchani@DFCI.HARVARD.EDU
lab head
Haopeng Xiao
contact affiliationDana-Farber Cancer Institute/Harvard Medical School
contact emailhaopeng_xiao@dfci.harvard.edu
dataset submitter
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Dataset FTP location
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