PXD029237-1

PXD029237 is an original dataset announced via ProteomeXchange.

Title	Coxsackievirus Adenovirus Receptor co-ordinates pro-inflammatory responses in the lung epithelium
Description	Airway inflammation and remodelling are key pathophysiologic features process in many respiratory conditions such as asthma. An intact epithelial cell layer is crucial to maintain lung homeostasis, and this depends on intercellular adhesion. The Coxsackievirus Adenovirus Receptor (CAR) is highly expressed in the epithelium where it modulates cell-cell adhesion stability and acts as a receptor for immune cells to facilitate transepithelial migration. Here we investigated the mechanistic role of CAR in mediating responses to the common aeroallergen House Dust Mite (HDM). We demonstrate that administration of HDM in mice lacking CAR in the respiratory epithelium leads to loss of peri-bronchial inflammatory cell infiltration, fewer goblet-cells, decreased IL-4 and IL-13 levels and reduced matrix remodelling. In vitro analysis in human lung epithelial cells confirmed that loss of CAR led to reduced HDM-dependent inflammatory cytokine release leading to reduced inflammatory cell transmigration. Moreover, CAR was required for HDM-induced TGF release leading to enhanced airway smooth muscle cell proliferation and matrix production. Our data demonstrates that CAR is a novel central co-ordinator of lung inflammation through a dual role in leukocyte recruitment and tissue remodelling and may represent an important target for future therapeutic development in lung inflammatory diseases.
HostingRepository	PRIDE
AnnounceDate	2022-09-20
AnnouncementXML	Submission_2022-09-20_07:01:44.952.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marieluise Kirchner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-20 03:06:01	ID requested
⏵ 1	2022-09-20 07:01:45	announced

Dataset with its publication pending

submitter keyword: BioID, CAR interactome, 16HBE human bronchial epithelial cells

Philipp Mertins
contact affiliation	Core Unit Proteomics, Berlin Institute of Health at Charite- Universitaetsmedizin Berlin and Max Delbrueck Center for Molecular Medicine (MDC), 13125 Berlin, Germany
contact email	Philipp.Mertins@mdc-berlin.de
lab head
Marieluise Kirchner
contact affiliation	Proteomics Platform, BIH@Charite
contact email	marieluise.kirchner@mdc-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029237
     1. Label: PRIDE project
     2. Name: Coxsackievirus Adenovirus Receptor co-ordinates pro-inflammatory responses in the lung epithelium