<<< Full experiment listing

PXD029144-1

PXD029144 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics and immunoproteomics of Anoplocephala perfoliata
DescriptionAnoplocephala perfoliata is a common tapeworm in horse with an ability to cause colic and increase mortality. At present, current diagnostic methods are not sensitive enough to detect A. perfoliata and immunoreactive excretory/secretory (E/S) proteins produced by this parasite, which comprise the most promising candidates for diagnostic tests. Here, we compared the E/S proteins produced by A. perfoliata worms of two different physiological states (small- and large-sized worms) in vitro by label-free quantitative (LFQ) proteomics with protein database composed of proteins encoded by Hymenolepis diminuta, Echinococcus multilocularis/granulosus and Taenia aseatica for protein identifications. The quantitative proteomics data was complemented with one-dimensional gel electrophoresis combined with immunoblotting using antisera from horses diagnosed positive and negative for this tapeworm. Protein bands cross-reacting most efficiently with the anti-A. perfoliata antibodies were subjected to in-gel digestion with LC-MS/MS identification. Both the non-gel and gel-based proteomic results were compared to propose a list of candidate proteins for further studies. These proteins will be validated for their suitability as diagnostic markers specific to cestodes and A. perfoliata.
HostingRepositoryPRIDE
AnnounceDate2022-11-11
AnnouncementXMLSubmission_2022-11-11_00:32:26.038.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTuulaNyman
SpeciesList scientific name: Anoplocephala perfoliata; NCBI TaxID: 218192;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-15 03:22:05ID requested
12022-11-11 00:32:26announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Perseus, E/S proteins, Tapeworm,Parasite, Horse, MaxQuant, LC-MS/MS, 1D immunoblotting
Contact List
TuulaNyman
contact affiliationHead of Proteomics
contact emailtuula.nyman@medisin.uio.no
lab head
TuulaNyman
contact affiliationUniversity of Oslo
contact emailtuula.nyman@medisin.uio.no
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD029144
PRIDE project URI
Repository Record List
[ + ]