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PXD029144

PXD029144 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics and immunoproteomics of Anoplocephala perfoliata
DescriptionAnoplocephala perfoliata is a common tapeworm in horse with an ability to cause colic and increase mortality. At present, current diagnostic methods are not sensitive enough to detect A. perfoliata and immunoreactive excretory/secretory (E/S) proteins produced by this parasite, which comprise the most promising candidates for diagnostic tests. Here, we compared the E/S proteins produced by A. perfoliata worms of two different physiological states (small- and large-sized worms) in vitro by label-free quantitative (LFQ) proteomics with protein database composed of proteins encoded by Hymenolepis diminuta, Echinococcus multilocularis/granulosus and Taenia aseatica for protein identifications. The quantitative proteomics data was complemented with one-dimensional gel electrophoresis combined with immunoblotting using antisera from horses diagnosed positive and negative for this tapeworm. Protein bands cross-reacting most efficiently with the anti-A. perfoliata antibodies were subjected to in-gel digestion with LC-MS/MS identification. Both the non-gel and gel-based proteomic results were compared to propose a list of candidate proteins for further studies. These proteins will be validated for their suitability as diagnostic markers specific to cestodes and A. perfoliata.
HostingRepositoryPRIDE
AnnounceDate2022-11-11
AnnouncementXMLSubmission_2022-11-11_00:32:26.038.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTuulaNyman
SpeciesList scientific name: Anoplocephala perfoliata; NCBI TaxID: 218192;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-15 03:22:05ID requested
12022-11-11 00:32:26announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Perseus, E/S proteins, Tapeworm,Parasite, Horse, MaxQuant, LC-MS/MS, 1D immunoblotting
Contact List
TuulaNyman
contact affiliationHead of Proteomics
contact emailtuula.nyman@medisin.uio.no
lab head
TuulaNyman
contact affiliationUniversity of Oslo
contact emailtuula.nyman@medisin.uio.no
dataset submitter
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Dataset FTP location
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