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PXD029120-1

PXD029120 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleG3BP1 inhibits Cul3SPOP to amplify AR signaling and promote prostate cancer
DescriptionSPOP, an E3 ubiquitin ligase, acts as a prostate-specific tumor suppressor with several key substrates mediating oncogenic function. However, the mechanisms underlying SPOP regulation are largely unknown. Here, we have identified G3BP1 as an interactor of SPOP and functions as a competitive inhibitor of Cul3SPOP, suggesting a distinctive mode of Cul3SPOP inactivation in prostate cancer (PCa). Transcriptomic analysis and functional studies reveal a G3BP1-SPOP ubiquitin signaling axis that promotes PCa progression through activating AR signaling. Moreover, AR directly upregulates G3BP1 transcription to further amplify G3BP1-SPOP signaling in a feed-forward manner. Our study supports a fundamental role of G3BP1 in disabling the tumor suppressive Cul3SPOP, thus defining a PCa cohort independent of SPOP mutation. Therefore, there are significantly more PCa that are defective for SPOP ubiquitin ligase than previously appreciated, and these G3BP1high PCa are more susceptible to AR-targeted therapy.
HostingRepositoryPRIDE
AnnounceDate2021-11-25
AnnouncementXMLSubmission_2021-11-25_06:59:18.624.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChandrani Mukhopadhyay
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-13 23:04:06ID requested
12021-11-25 06:59:19announced
Publication List
Dataset with its publication pending
Keyword List
ProteomeXchange project tag: Human Proteome Project
submitter keyword: TAP-MS, Human, Prostate Cancer, SPOP, G3BP1
Contact List
Pengbo Zhou
contact affiliationDepartment of Pathology and Laboratory Medicine, The Joan and Stanford I. Weill Medical College of Cornell University, New York, NY 10065, USA
contact emailpez2001@med.cornell.edu
lab head
Chandrani Mukhopadhyay
contact affiliationWeill Cornell Medicine
contact emailchm2067@med.cornell.edu
dataset submitter
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Dataset FTP location
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