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PXD028963-1

PXD028963 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain
DescriptionIn order to combat molecular damage, most cellular proteins undergo rapid turnover. We have previously identified large nuclear protein assemblies that can persist for years in post-mitotic tissues and are subject to age-related decline. Here we report that mitochondria can be long-lived in the mouse brain and reveal that specific mitochondrial proteins have half-lives longer than the average proteome. These mitochondrial long-lived proteins (mitoLLPs) are core components of the electron transport chain (ETC) and display increased longevity in respiratory supercomplexes. We find that COX7C, a mitoLLP that forms a stable contact site between Complexes I and IV, is required for Complex IV and supercomplex assembly. Remarkably, even upon depletion of COX7C transcripts, ETC function is maintained for days, effectively uncoupling mitochondrial function from ongoing transcription of its mitoLLPs. Our results suggest that modulating protein longevity within the ETC is critical for mitochondrial proteome maintenance and the robustness of mitochondrial function.
HostingRepositoryPRIDE
AnnounceDate2021-11-04
AnnouncementXMLSubmission_2021-11-04_05:07:05.960.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD028963
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterShefali Krishna
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList6x(13)C labeled residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-06 06:05:08ID requested
12021-11-04 05:07:06announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Mitochondria
protein homeostasis
long-lived proteins
electron transport chain
Contact List
Martin Hetzer
contact affiliationSalk Institute, La Jolla, CA USA
contact emailhetzer@salk.edu
lab head
Shefali Krishna
contact affiliationSalk Institute of Biological Studies
contact emailskrishna@salk.edu
dataset submitter
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Dataset FTP location
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