PXD028909 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | TST knockout liver proteome iTRAQ and persulfidome |
Description | Impaired hepatic glucose and lipid metabolism are hallmarks of type–2 diabetes. Increased sulfide production or sulfide–donor compounds may beneficially regulate hepatic metabolism. Disposal of sulfide through the sulfide oxidation pathway (SOP) is critical for maintaining sulfide within a safe physiological range. We show that mice lacking the liver–enriched mitochondrial SOP enzyme thiosulfate sulfur–transferase (Tst—/— mice) exhibit high circulating sulfide, increased gluconeogenesis, hypertriglyceridemia and fatty liver. Unexpectedly, hepatic sulfide levels are normal in Tst—/— mice due to exaggerated induction of sulfide disposal, with an associated suppression of global protein persulfidation and nuclear respiratory factor–2 target protein levels. Hepatic proteomic and persulfidomic profiles converge on gluconeogenesis and lipid metabolism, revealing a selective deficit in medium–chain fatty acid oxidation in Tst—/— mice. We reveal a critical role for TST in hepatic metabolism that has implications for sulfide-donor strategies in the context of metabolic disease. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-11 |
AnnouncementXML | Submission_2021-11-11_08:46:03.448.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Pauline Ward |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | sulfated residue; iTRAQ4plex-116 reporter+balance reagent acylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-10-04 04:45:48 | ID requested | |
⏵ 1 | 2021-11-11 08:46:03 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Liver, Mouse, TST, Proteome, Persulfidome, MSMS |
Contact List
Nik Morton |
contact affiliation | University of Edinburgh |
contact email | Nik.Morton@ed.ac.uk |
lab head | |
Pauline Ward |
contact affiliation | University of Edinburgh |
contact email | pauline.ward@ed.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD028909 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028909
- Label: PRIDE project
- Name: TST knockout liver proteome iTRAQ and persulfidome