PXD028871-2
PXD028871 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | CoolTip: Low-Temperature Solid-Phase Microextraction Tip for Capturing Hydrophilic Phosphopeptides: 2 |
Description | We developed a simple temperature controlled desalting strategy to cover from hydrophilic to hydrophobic peptides. Reversed-phase solid-phase extraction (SPE) techniques are commonly used for shotgun-proteomics using liquid chromatography/tandem mass spectrometry. In reversed-phase chromatography, solid-phase temperature is one of the parameters that influences peptide retention, and the analytical column temperatures have been well optimized for the peptide separations. However, little attention has been given to the temperatures of solid-phase extraction columns for sample preparations. We showed that the recovery of hydrophilic (phospho)peptides improved with lower temperature SPE-tips (CoolTips) and identification coverage of the hydrophilic (phospho)peptides can be extended without sacrificing the recovery of hydrophobic peptides. CoolTip also provided better quantitative reproducibility of the peptides, and we envision this protocol can improve the performance of any kinds of proteomics experiments. |
HostingRepository | jPOST |
AnnounceDate | 2021-10-28 |
AnnouncementXML | Submission_2021-11-07_17:00:26.863.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kosuke Ogata |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-09-30 02:43:56 | ID requested | |
1 | 2021-10-28 17:53:03 | announced | |
⏵ 2 | 2021-11-07 17:00:27 | announced | 2021-11-08: Updated PubMed. |
3 | 2022-09-18 03:04:22 | announced | 2022-09-18: Updated FTP location. |
Publication List
Ogata K, Ishihama Y, CoolTip: Low-Temperature Solid-Phase Extraction Microcolumn for Capturing Hydrophilic Peptides and Phosphopeptides. Mol Cell Proteomics, 20():100170(2021) [pubmed] |
Keyword List
submitter keyword: CoolTip, Phosphoproteomics |
Contact List
Yasushi Ishihama | |
---|---|
lab head | |
Kosuke Ogata | |
contact affiliation | Kyoto university |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST001344 |