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PXD028824-1

PXD028824 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDOT1L regulates pericentromeric heterochromatin
DescriptionMany repetitive DNA elements are packaged in heterochromatin, but depend on occasional transcription to maintain long-term silencing. The factors that promote transcription of repeat elements in heterochromatin are largely unknown. Here, we show that DOT1L, a histone methyltransferase that modifies lysine 79 of histone H3 (H3K79), is required for transcription of major satellite repeats to maintain pericentromeric heterochromatin (PCH), and that this function is essential for preimplantation development. DOT1L is a transcriptional activator at single-copy genes but does not have a known role in repeat element transcription. We show that H3K79me3 is specifically enriched at repetitive elements, that loss of DOT1L compromises pericentromeric major satellite transcription, and that this function depends on interaction between DOT1L and the chromatin remodeler SMARCA5. DOT1L inhibition causes chromosome breaks and cell cycle defects, and leads to embryonic lethality. Together, our findings uncover a vital new role for DOT1L in transcriptional activation of heterochromatic repeats.
HostingRepositoryPRIDE
AnnounceDate2023-05-26
AnnouncementXMLSubmission_2023-05-26_08:32:05.939.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTuKietLam
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-29 00:11:27ID requested
12023-05-26 08:32:06announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: mESCs, embryo, mass spectrometry,DOT1L, chromatin, pericentromere
Contact List
TuKietLam
contact affiliationYale University Department of Molecular Biophysics and Biochemistry
contact emailtukiet.lam@yale.edu
lab head
TuKietLam
contact affiliationYale University
contact emailtukiet.lam@yale.edu
dataset submitter
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Dataset FTP location
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