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PXD028727-1

PXD028727 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDeciphering the molecular mechanism of long non-coding RNA HIFA-AS1 regulating pancreatic cancer cells
DescriptionHIFA-AS1, an antisense transcript of HIF1α gene, is a 652-bp long non-coding RNA (lncRNA) which globally expressed in multiple tissues of animals. Recent evidence indicated that the HIFA-AS1 was involved in tumorigenesis of several types of cancer, but there were no reports on pancreatic cancer (PC). In order to investigate whether the HIFA-AS1 could mediate the PC or not, it was overexpressed in a PC cell line (PANC-1), and a series of experiments including cell viability detection, flow cytometry, and transwell migration were performed. Functionally, the results indicated that overexpression (OE) of HIFA-AS1 could inhibit proliferation and shift, and promote apoptosis of PC cells. Moreover, to explore underlying molecular mechanism of HIFA-AS1 in PC cells, the iTRAQ (isobaric tags for relative and absolute quantification) quantitative proteomics analysis was implemented. Mechanistically, it was found that OE of HIFA-AS1 globally affected the expression levels of multiple protein associated with metabolism of cancer, suggesting that HIFA-AS1 might regulate the tumorigenesis of PC by up- and down-regulating the gene expression.
HostingRepositoryiProX
AnnounceDate2021-09-23
AnnouncementXMLSubmission_2021-09-23_19:29:30.887.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHe Zhang
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-23 19:28:47ID requested
12021-09-23 19:29:31announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: HIFA-AS1, iTRAQ
Contact List
Jing Tian
contact affiliationGeneral Hospital of Northern Theater Command, China
contact email13352459336@163.com
lab head
He Zhang
contact affiliationGeneral Hospital of Northern Theater Command, China
contact emailhzauzhoujian@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI