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PXD028629-1

PXD028629 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNorthern elephant seal skeletal muscle, blubber, and plasma proteome responses to prolonged fasting
DescriptionNorthern elephant seal skeletal muscle, blubber, and plasma were collected from 5 adult females early and late in a 5-week molting fast (mean 33 days apart; see Sample Key in supplementary files). Proteins were isolated from muscle using the Trizol method and blubber using Qiazol with the Qiagen RNeasy Lipid Mini kit (see Khudyakov et al., 2018, doi: 10.1242/bio.036731). Plasma samples were used raw (without depletion of highly abundant proteins). Muscle, blubber, and plasma proteins were denatured using 1% sodium deoxycholate, 8 M urea, and 5 mM DTT in 50 mM ammonium bicarbonate and digested in-solution using trypsin (1:50 trypsin to protein ratio).
HostingRepositoryMassIVE
AnnounceDate2021-09-19
AnnouncementXMLSubmission_2021-09-19_15:16:34.239.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJane Khudyakov
SpeciesList scientific name: Mirounga angustirostris; common name: Northern elephant seal; NCBI TaxID: 9716;
ModificationListCarbamidomethyl; Oxidation; Deamidated
InstrumentOrbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-19 14:28:07ID requested
12021-09-19 15:16:34announced
Publication List
no publication
Keyword List
submitter keyword: fasting, proteome, seal, muscle, blubber, plasma
Contact List
Jane Khudyakov
contact affiliationUniversity of the Pacific
contact emailjkhudyakov@pacific.edu
lab head
Jane Khudyakov
contact affiliationUniversity of the Pacific
contact emailjkhudyakov@pacific.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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